刘彦梅，博士，研究员，博士生导师。本科与硕士毕业于北京大学医学部临床医学专业，在新加坡国立大学获得生物学博士学位，曾于德国马克斯普朗克研究所/德累斯顿工业大学医学院做博士后，北京大学分子医学研究所任副研究员，2018年9月以青年拔尖人才引进方式加盟华南师范大学脑科学与康复医学研究院。长期致力于胰岛细胞发育与再生机制的研究。近年来研究兴趣扩展为改造和应用基因编辑技术构建单基因脑疾病的斑马鱼模型，并利用斑马鱼脑疾病模型进行病理机制研究以及药物筛选。其一系列研究成果以第一作者或(共)通讯作者发表在《Blood》、《Nature Communications》、《Developmental Cell》、《Elife》、《Diabetes Obesity and Metabolism》、《Journal of Cell Science》等杂志。
代表论著：
第一及（共）通讯作者文章：
1. Resolving vertebrate brain evolution through salamander brain development and regeneration. Pan XY, Zeng YY, Liu YM*, Fei JF*. Zool Res. 2023 Jan 18;44(1):219-222. doi: 10.24272/j.issn.2095-8137.2022.527.
2. Wang L, Zeng YY, Liu Y*, Fei JF*. Applying a Knock-In Strategy to Create Reporter-Tagged Knockout Alleles in Axolotls. Methods Mol Biol. 2023; 2562:351-368. doi:10.1007/978-1-0716-2659-7_23.
3. Liang F, Zhang Y, Li L, Yang Y, Fei JF, Liu Y*, Qin W*. SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish. Nat Commun. 2022 Jun 14;13(1):3421. doi:10.1038/s41467-022-31034-8.
4. 彭程, 刘彦梅*, 费继锋* 神经环路再生研究进展. 中国细胞生物学学报 Chinese Journal of Cell Biology 2022, 44(8): 1529–1541 DOI: 10.11844/cjcb.2022.08.0009
5. Zhang Y, Han C, Zhu W, Yang G, Peng X, Mehta S, Zhang J, Chen L*, Liu Y*. Glucagon Potentiates Insulin Secretion Via β-Cell GCGR at Physiological Concentrations of Glucose. Cells 2021, 10, 2495. https://doi.org/10.3390/ cells10092495
6. Zhao J, Liang S, Zhao Y, Zong W, Tran E, Chen L, Liu Y. In vivo Imaging of Calcium Activities from Pancreatic β-cells in Zebrafish Embryos Using Spinning-disc Confocal and Two-photon Light-sheet Microscopy. Bio Protoc. 2021 Dec 5;11(23):e4245. doi: 10.21769/BioProtoc.4245. eCollection 2021 Dec 5. PMID: 35005090
7. Liu Y, Lou W, Fei JF. The engine initiating tissue regeneration: does a common mechanism exist during evolution? Cell Regen. 2021 Apr 5;10(1):12. doi: 10.1186/s13619-020-00073-1.
8. Kang F, Zhou M, Huang X, Fan J, Wei L, Boulanger J, Liu Z, Salamero J, Liu Y*, Chen L*. E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment. Sci Rep. 2019 Mar 8;9(1):3975. doi: 10.1038/s41598-019-40331-0.
9. Zhao J, Zong W, Zhao Y, Gou D, Liang S, Shen J, Wu Y, Zheng X, Wu R, Wang X, Niu F, Wang A, Zhang Y, Xiong JW, Chen L*, Liu Y*. In Vivo imaging of b-cell function reveals glucose-mediated heterogeneity of b-cell functional development. Elife. 2019 Jan 29;8. pii: e41540. doi: 10.7554/eLife.41540.
Highlighted by a Research Highlight in Nature Reviews Endocrinology (https://www.nature.com/articles/s41574-019-0181-y).
10. Wang Y, Han C, Zhu W, Wu Z, Liu Y*, Chen L*. An optical method to evaluate both mass and functional competence of pancreatic alpha- and beta-cells. Journal of Cell Science. 2016;129(12):2462-71. doi: 10.1242/jcs.184523. (Cover story)
11. Yuan T, Liu L, Zhang Y, Wei L, Zhao S, Zheng X, Huang X, Boulanger J, Gueudry C, Lu J, Xie L, Du W, Zong W, Yang L, Salamero J, Liu Y*, Chen L*. Diacylglycerol guides the hopping of clathrin-coated pits along microtubules for exo-endocytosis coupling. Developmental Cell. 2015;35(1):120-30.
Previewed in the same issue of Dev Cell, and highlighted in the F1000Prime and also at the American Society of Cell Biology (ASCB) website.
12. 刘彦梅*, 郑晓璐，袁天一，陈良怡* 可兴奋细胞胞吐胞吞的耦联机制。中国细胞生物学学报 2015; (12):1606-15.
13. Liu Y, Suckale J, Masjkur J, Magro MG, Steffen A, Anastassiadis K, Solimena M. Tamoxifen-independent recombination in the RIP-CreER mouse. PLoS One. 2010 Oct 22;5(10):e13533.
14. Liu Y, Mziaut H, Ivanova A, Solimena M.beta-Cells at the crossroads: choosing between insulin granule production and proliferation. Diabetes Obes Metab. 2009 Nov;11 Suppl 4:54-64.
15. Liu Y, Du L, Osato M, Teo EH, Qian F, Jin H, Zhen F, Xu J, Guo L, Huang H, Chen J, Geisler R, Jiang YJ, Peng J, Wen Z. The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development. Blood. 2007 Jul 1;110(1):99-106.
合作作者文章：
1. Hu Y, Pan X, Shi Y, Qiu Y, Wang L, Murawala P, Liu Y, Xing W, Tanaka EM, Fei JF. Muscles are barely required for the patterning and cell dynamics in axolotl limb regeneration. Front Genet. 2022 Oct 10;13:1036641. doi: 10.3389/fgene.2022.1036641. eCollection 2022.
2. Zhao W, Zhao S, Li L, Huang X, Xing S, Zhang Y, Qiu G, Han Z, Shang Y, Sun DE, Shan C, Wu R, Gu L, Zhang S, Chen R, Xiao J, Mo Y, Wang J, Ji W, Chen X, Ding B, Liu Y, Mao H, Song BL, Tan J, Liu J, Li H, Chen L. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy. Nat Biotechnol. 2021 Nov 15. doi: 10.1038/s41587-021-01092-2. Online ahead of print. PMID: 34782739
3. Yang G, Li L, Liu Y, Liang K, Wei L, Chen L. Hyperglycemia-Induced Dysregulated Fusion Intermediates in Insulin-Secreting Cells Visualized by Super-Resolution Microscopy. Front Cell Dev Biol. 2021 Apr 15;9:650167. doi: 10.3389/fcell.2021.650167. eCollection 2021. PMID: 33937248
4. Dong D, Huang X, Li L, Mao H, Mo Y, Zhang G, Zhang Z, Shen J, Liu W, Wu Z, Liu G, Liu Y, Yang H, Gong Q, Shi K, Chen L. Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome. Light Sci Appl. 2020 Jan 28;9:11. doi: 10.1038/s41377-020-0249-4. eCollection 2020.
5. Zhang Y, Wang J, Xing S, Li L, Zhao S, Zhu W, Liang K, Liu Y, Chen L. Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging. Sci China Life Sci. 2020 Oct;63(10):1543-1551. doi: 10.1007/s11427-019-1659-4. Epub 2020 Apr 8.
6. Huang X, Fan J, Li L, Liu H, Wu R, Wu Y, Wei L, Mao H, Lal A, Xi P, Tang L, Zhang Y, Liu Y, Tan S*, Chen L*. Ultrasensitive Hessian structured illumination microscopy enables ultrafast and long-term super-resolution imaging. Nature Biotechnology. 2018; 36(5):451-459. doi: 10.1038/nbt.4115.
7. Ivanova A, Kalaidzidis Y, Dirkx R, Sarov M, Gerlach M, Schroth-Diez B, Müller A, Liu Y, Andree C, Mulligan B, Münster C, Kurth T, Bickle M, Speier S, Anastassiadis K, Solimena M. Age-dependent labeling and imaging of insulin secretory granules. Diabetes. 2013 Nov;62(11):3687-96.
8. Du L, Xu J, Li X, Ma N, Liu Y, Peng J, Osato M, Zhang W, Wen Z. Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development. 2011 Feb;138(4):619-29.
授权发明专利:
刘彦梅，赵佳，陈良怡，“一种在体筛选促胰岛β细胞功能药物的方法”（专利号ZL 201710010182.4）。专利申请日：2017年1月6日，授权公告日：2021年9月14日，授权公告号：CN 106755099 B
承担科研项目情况：
1. 国家自然科学基金面上项目（32070819）：活体实时成像研究胰岛三种内分泌细胞协同发育和获得功能的机制, 2021/01-2024/12，主持
2. 深圳华大生命科学研究院开放基金项目（BGIRSZ20210002）：结合时空组学技术研究蝾螈胰岛β细胞再生机制，2022/01-2023/12，主持
3. 国家重点研发计划国际合作重点专项（2019YFE0106700）：应用墨西哥钝口螈鉴定直接参与组织再生的细胞类型及其启动再生的关键因子，2020/07-2022/06，骨干
4. 广东省科技厅农村科技特派员项目（KTP20200110）：水生动物的标准化养殖，2020/07-2022/06，骨干
5. 华南师范大学高水平大学建设专项资金高层次引进人才科研启动经费, 2018/09-2026/08，主持
6. 国家自然科学基金重大研究计划培育项目(91854112)： 应用海森结构光超高分辨率显微镜研究靶向溶酶体的线粒体衍生囊泡的发生机制，2019/01-2021/12，主持
7. 国家自然科学基金重大研究计划重点项目（91750203）：基于光场调控的光学成像新技术与应用研究，2018/01-2021/12，骨干
8. 北京市自然科学基金面上项目（7152079）：瑞特综合征致病因子MeCP2调控胰岛细胞重新编程的机制及其在糖尿病发病中的作用，2015/01-2017/12，主持
9. 国家自然科学基金青年项目（31301186）：利用斑马鱼在体荧光成像研究胰岛beta 细胞的分泌和成熟机制，2014/01-2016/12，主持
欢迎志同道合的硕士生、博士生、博士后及青年英才的加盟,让我们在科研道路上一起携手,不忘初心,砥砺前行! 联系邮箱：yanmeiliu@m.scnu.edu.cn